Eva LaRue as Natalia Boa Vista an undercover FBI Agent assigned to the team.Rex Linn as Frank Tripp a senior Robbery-Homicide Division (RHD) Detective assigned to assist the CSI's.
Jonathan Togo as Ryan Wolfe a newly hired CSI Detective and Delko's partner.
Khandi Alexander as Alexx Woods a Medical Examiner assigned to CSI.Adam Rodriguez as Eric Delko a CSI Detective and Wolfe's partner.Emily Procter as Calleigh Duquesne a veteran CSI Detective, the CSI Assistant Supervisor and a ballistics expert.David Caruso as Horatio Caine a CSI Lieutenant and the Director of the MDPD Crime Lab.With Alexx accused of murdering a child molester, Horatio accused of murdering an innocent woman, Wolfe facing the loss of his eye, and Mac Taylor joining the team when a prisoner transfer goes awry, it's ultimately Delko who faces the greatest loss: his sister who was to marry Horatio is gunned down by the Mala Noche gang in an attack that will begin a narrative recurring throughout the next several seasons.
Under the roof of a state-of-the-art redesigned crime lab, the dynamic duo investigate a series of crimes including a night-club murder, the death of a pool boy, a cold case rape, and a murder with a double-jeopardy clause, all whilst under the scrutiny of an anonymous mole, and facing a series of cases that threaten the team. With a mole in their midst, Horatio and Calleigh head a team of elite Crime Scene Investigators as they enter their most challenging season yet.
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